Co‐localization of Middle East respiratory syndrome coronavirus (MERS‐CoV) and dipeptidyl peptidase‐4 in the respiratory tract and lymphoid tissues of pigs and llamas
Identifieur interne : 000A20 ( Main/Exploration ); précédent : 000A19; suivant : 000A21Co‐localization of Middle East respiratory syndrome coronavirus (MERS‐CoV) and dipeptidyl peptidase‐4 in the respiratory tract and lymphoid tissues of pigs and llamas
Auteurs : Nigeer Te ; Júlia Vergara-Alert ; Annika Lehmbecker ; M Nica Pérez ; Bart L. Haagmans ; Wolfgang Baumg Rtner ; Albert Bensaid ; Joaquim SegalésSource :
- Transboundary and Emerging Diseases [ 1865-1674 ] ; 2018.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Animaux, Appareil respiratoire (enzymologie), Camélidés du Nouveau Monde (virologie), Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité), Dipeptidyl peptidase 4 (métabolisme), Immunohistochimie (médecine vétérinaire), Infections à coronavirus (médecine vétérinaire), Infections à coronavirus (virologie), Maladies des porcs (virologie), Microscopie électronique à balayage (médecine vétérinaire), Réaction de polymérisation en chaine en temps réel (médecine vétérinaire), Récepteurs viraux (métabolisme), Suidae, Tissu lymphoïde (enzymologie).
- MESH :
- enzymologie : Appareil respiratoire, Tissu lymphoïde.
- génétique : ARN viral.
- médecine vétérinaire : Immunohistochimie, Infections à coronavirus, Microscopie électronique à balayage, Réaction de polymérisation en chaine en temps réel.
- métabolisme : Dipeptidyl peptidase 4, Récepteurs viraux.
- pathogénicité : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Camélidés du Nouveau Monde, Infections à coronavirus, Maladies des porcs.
- Animaux, Suidae.
English descriptors
- KwdEn :
- Animals, Camelids, New World (virology), Coronavirus Infections (veterinary), Coronavirus Infections (virology), Dipeptidyl Peptidase 4 (metabolism), Immunohistochemistry (veterinary), Lymphoid Tissue (enzymology), Microscopy, Electron, Scanning (veterinary), Middle East Respiratory Syndrome Coronavirus (pathogenicity), RNA, Viral (genetics), Real-Time Polymerase Chain Reaction (veterinary), Receptors, Virus (metabolism), Respiratory System (enzymology), Swine, Swine Diseases (virology).
- MESH :
- chemical , genetics : RNA, Viral.
- chemical , metabolism : Dipeptidyl Peptidase 4, Receptors, Virus.
- enzymology : Lymphoid Tissue, Respiratory System.
- pathogenicity : Middle East Respiratory Syndrome Coronavirus.
- veterinary : Coronavirus Infections, Immunohistochemistry, Microscopy, Electron, Scanning, Real-Time Polymerase Chain Reaction.
- virology : Camelids, New World, Coronavirus Infections, Swine Diseases.
- Animals, Swine.
Abstract
This study investigated the co‐localization of the Middle East respiratory syndrome coronavirus (
Url:
DOI: 10.1111/tbed.13092
PubMed: 30520548
PubMed Central: 7027813
Affiliations:
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Le document en format XML
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Haagmans</name><affiliation><nlm:aff id="tbed13092-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Baumg Rtner, Wolfgang" sort="Baumg Rtner, Wolfgang" uniqKey="Baumg Rtner W" first="Wolfgang" last="Baumg Rtner">Wolfgang Baumg Rtner</name><affiliation><nlm:aff id="tbed13092-aff-0002"></nlm:aff></affiliation></author><author><name sortKey="Bensaid, Albert" sort="Bensaid, Albert" uniqKey="Bensaid A" first="Albert" last="Bensaid">Albert Bensaid</name><affiliation><nlm:aff id="tbed13092-aff-0001"></nlm:aff></affiliation></author><author><name sortKey="Segales, Joaquim" sort="Segales, Joaquim" uniqKey="Segales J" first="Joaquim" last="Segalés">Joaquim Segalés</name><affiliation><nlm:aff id="tbed13092-aff-0004"></nlm:aff></affiliation><affiliation><nlm:aff id="tbed13092-aff-0005"></nlm:aff></affiliation></author></analytic><series><title level="j">Transboundary and Emerging Diseases</title><idno type="ISSN">1865-1674</idno><idno type="eISSN">1865-1682</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Camelids, New World (virology)</term><term>Coronavirus Infections (veterinary)</term><term>Coronavirus Infections (virology)</term><term>Dipeptidyl Peptidase 4 (metabolism)</term><term>Immunohistochemistry (veterinary)</term><term>Lymphoid Tissue (enzymology)</term><term>Microscopy, Electron, Scanning (veterinary)</term><term>Middle East Respiratory Syndrome Coronavirus (pathogenicity)</term><term>RNA, Viral (genetics)</term><term>Real-Time Polymerase Chain Reaction (veterinary)</term><term>Receptors, Virus (metabolism)</term><term>Respiratory System (enzymology)</term><term>Swine</term><term>Swine Diseases (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>Animaux</term><term>Appareil respiratoire (enzymologie)</term><term>Camélidés du Nouveau Monde (virologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité)</term><term>Dipeptidyl peptidase 4 (métabolisme)</term><term>Immunohistochimie (médecine vétérinaire)</term><term>Infections à coronavirus (médecine vétérinaire)</term><term>Infections à coronavirus (virologie)</term><term>Maladies des porcs (virologie)</term><term>Microscopie électronique à balayage (médecine vétérinaire)</term><term>Réaction de polymérisation en chaine en temps réel (médecine vétérinaire)</term><term>Récepteurs viraux (métabolisme)</term><term>Suidae</term><term>Tissu lymphoïde (enzymologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Dipeptidyl Peptidase 4</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Appareil respiratoire</term><term>Tissu lymphoïde</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Lymphoid Tissue</term><term>Respiratory System</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Immunohistochimie</term><term>Infections à coronavirus</term><term>Microscopie électronique à balayage</term><term>Réaction de polymérisation en chaine en temps réel</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dipeptidyl peptidase 4</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term><term>Immunohistochemistry</term><term>Microscopy, Electron, Scanning</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Camélidés du Nouveau Monde</term><term>Infections à coronavirus</term><term>Maladies des porcs</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Camelids, New World</term><term>Coronavirus Infections</term><term>Swine Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Suidae</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p>This study investigated the co‐localization of the Middle East respiratory syndrome coronavirus (<styled-content style="fixed-case" toggle="no">MERS</styled-content>‐CoV) and its receptor dipeptidyl peptidase‐4 (<styled-content style="fixed-case" toggle="no">DPP</styled-content>4) by immunohistochemistry (IHC) across respiratory and lymphoid organs of experimentally <styled-content style="fixed-case" toggle="no">MERS</styled-content>‐CoV infected pigs and llamas. Also, scanning electron microscopy was performed to assess the ciliary integrity of respiratory epithelial cells in both species. In pigs, on day 2 post‐inoculation (p.i.), <styled-content style="fixed-case" toggle="no">DPP</styled-content>4‐<styled-content style="fixed-case" toggle="no">MERS</styled-content>‐CoV co‐localization was detected in medial turbinate epithelium. On day 4 p.i., the virus/receptor co‐localized in frontal and medial turbinate epithelial cells in pigs, and epithelial cells distributed unevenly through the whole nasal cavity and in the cervical lymph node in llamas. <styled-content style="fixed-case" toggle="no">MERS</styled-content>‐CoV viral nucleocapsid was mainly detected in upper respiratory tract sites on days 2 and 4 p.i. in pigs and day 4 p.i. in llamas. No <styled-content style="fixed-case" toggle="no">MERS</styled-content>‐CoV was detected on day 24 p.i. in any tissue by <styled-content style="fixed-case" toggle="no">IHC</styled-content>. While pigs showed severe ciliary loss in the nasal mucosa both on days 2 and 4 p.i. and moderate loss in the trachea on days 4 and 24 p.i., ciliation of respiratory organs in llamas was not significantly affected. Obtained data confirm the role of <styled-content style="fixed-case" toggle="no">DPP</styled-content>4 for <styled-content style="fixed-case" toggle="no">MERS</styled-content>‐CoV entry in respiratory epithelial cells of llamas. Notably, several nasal epithelial cells in pigs were found to express viral antigen but not <styled-content style="fixed-case" toggle="no">DPP</styled-content>4, suggesting the possible existence of other molecule/s facilitating virus entry or down regulation of <styled-content style="fixed-case" toggle="no">DPP</styled-content>4 upon infection.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Baumg Rtner, Wolfgang" sort="Baumg Rtner, Wolfgang" uniqKey="Baumg Rtner W" first="Wolfgang" last="Baumg Rtner">Wolfgang Baumg Rtner</name><name sortKey="Bensaid, Albert" sort="Bensaid, Albert" uniqKey="Bensaid A" first="Albert" last="Bensaid">Albert Bensaid</name><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L." last="Haagmans">Bart L. Haagmans</name><name sortKey="Lehmbecker, Annika" sort="Lehmbecker, Annika" uniqKey="Lehmbecker A" first="Annika" last="Lehmbecker">Annika Lehmbecker</name><name sortKey="Perez, M Nica" sort="Perez, M Nica" uniqKey="Perez M" first="M Nica" last="Pérez">M Nica Pérez</name><name sortKey="Segales, Joaquim" sort="Segales, Joaquim" uniqKey="Segales J" first="Joaquim" last="Segalés">Joaquim Segalés</name><name sortKey="Te, Nigeer" sort="Te, Nigeer" uniqKey="Te N" first="Nigeer" last="Te">Nigeer Te</name><name sortKey="Vergara Lert, Julia" sort="Vergara Lert, Julia" uniqKey="Vergara Lert J" first="Júlia" last="Vergara-Alert">Júlia Vergara-Alert</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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